The mouse monoclonal antibody clone AD2 binds to human CD73, a 70 kDa glycosylphosphatidylinositol (GPI)-linked 5’-nucleotidase. CD73, also known as ecto-5’-nucleotidase, has an enzymatic activity to convert extracellular adenosine 5’-monophosphate (AMP) to adenosine. It is present in subsets of both T and B cells and is found at a high level on B cells. It is also expressed in mesenchymal stem cells, follicular dendritic cells, epithelial cells, and endothelial cells. CD73 is also a novel immune checkpoint associated with adenosine metabolism whose expression may promote tumor progression by suppressing antitumor immune response and promoting angiogenesis. CD73 expression level may be a marker of poor cancer prognosis.

PRODUCT DETAILS

Clone: AD2

Specificity: CD73

Reactivity: Human

Format: cFluor^® R685

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Nakamura T, Kubagawa H, et al. 1993. J. Immunol. 151:6933-694
2. Knapp W. et al, 1989. Leucocyte typing IV Oxford University Press. p628-634.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® R685 is equivalent to CF^®660C manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.