
cFluor® R685 Anti-Human CD45 (HI30)
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Description
The mouse monoclonal antibody clone HI30 binds human CD45. Human CD45, also known as Leukocyte Common Antigen (LCA), is one of the most abundant hematopoietic markers. It is a 180-220 kDa single-chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except erythrocytes, platelets, and non-hematopoietic cells. Specifically, CD45 is found on the surface of T and B lymphocytes, natural killer cells, monocytes, macrophages, dendritic cells, neutrophils, eosinophils, and basophils. CD45 functions as a protein tyrosine phosphatase with several isoforms, each generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 plays a critical role in many leukocyte functions by regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45 with more restricted cellular expression include CD45R (B220), CD45RA, CD45RB, and CD45RO. It is reported that clones HI30 and 2D1 anti-human CD45 clones can reciprocally block the binding of each other to CD45.
PRODUCT DETAILS
Clone: HI30
Specificity: CD45
Reactivity: Human, Chimpanzee
Format: cFluor® R685*
Isotype: Mouse IgG1, κ
Tested Dilution: 5 µL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Gadhoum SZ, et al. 2008. Nat Chem Biol. 4:751-757.
- Lund-Johansen F, et al. 1992. J Immunol. 148:3221-3229
For research use only. Not intended for use in diagnostic procedures.
*cFluor® R685 is equivalent to CF®660C manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.