cFluor® R685 Anti-Human CD14 (MEM-18)
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Description
The mouse monoclonal antibody clone MEM-18 binds to human CD14, a 53-55-kDa glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that works as a receptor on myeloid cells for ligands such as lipopolysaccharide (LPS). It is found that CD14 is a receptor for and binds to complexes of LPS and LBP with high affinity. It is expressed on monocytes and macrophages at high levels. It is also present in some interfollicular dendritic cells, macrophages, reticular dendritic cells, and Langerhans cells.
PRODUCT DETAILS
Clone: MEM-18
Specificity: CD14
Reactivity: Human
Format: cFluor® R685*
Isotype: Mouse IgG1, κ
Test Dilution: 5 µl/Test
Application: Flow Cytometry
Storage: 2-8°C protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
Pugin J, et al. 1998. Infect Immun. 66:1174
Wright SD, et al. 1990. Science. 249:1431
For Research Use Only. Not intended for use in diagnostic procedures.
*cFluor® R685 is equivalent to CF®660C manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.