HNK-1 monoclonal antibody binds to human CD57, a 100-115 kDa oligosaccharide antigen expressed by several cell lineages¹. CD57, also known as HNK-1, NK-1, Leu-7 and L2, is present on peripheral blood lymphocyte subsets, including CD8 T cells and NK cells, neural and striated muscle cells^2,3. CD57 antigens are not expressed on erythrocytes, platelets, granulocytes, and monocytes⁴. While the function of CD57 is not well known, studies have shown that it may play a role in neural adhesion and cell-matrix interactions⁵. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  HNK-1

Reactivity:          Human, African Green, Baboon, Cynomolgus, Rhesus

Format:               cFluor® R668*

Isotype:               Mouse IgM, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:      TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Khan A, et al. 1998. Virchows Arch. 432(5):427-32
2. Kared H, et al. 2016. Cancer Immunol Immunother. 65(4):441-52
3. Mechtersheimer G, et al. 1991. Cancer Res. 51(4):1300-7
4. Stelin, S. et al. 2009. J Indian Soc Periodontol 13, 150-4.
5. Künemund V, et al. J Cell Biol. 1988.106(1):213-23

For Research Use Only. Not intended for use in diagnostic procedures.

*cFluor® R668 is equivalent to CF^®647 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.