The L161 monoclonal antibody binds to human, CD1c, a 43-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. CD1c, also known as BDCA-1, R7 or M241, is structurally related to MHC class I, forms heterodimers with beta-2 microglobulin¹. CD1s is an antigen-presenting protein that presents glycolipids to T cell^2,3. The molecule is expressed on thymocytes, dendritic cells, Langerhans cells, monocytes, mature T cells and circulating and marginal zone B cells. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DATA

Human peripheral blood was stained with CD19-BV510 and cFluor™ R668 Anti-Human CD1c (clone L161) (left) or cFluor™ R668 mouse IgG1, κ isotype control (right). Data shown is gated on lymphocytes.

PRODUCT DETAILS

Clone:                  L161

Reactivity:          Human

Format:                cFluor™ R668

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:      TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Bauer A, et al. 1997. Eur J Immunol. (6):1366-73
2. Adams EJ, et al. 2013. Mol Immunol. 55(2):182-5
3. Grant EP, et al. 1999. J Exp Med. 4;189(1):195-205

For Research Use Only. Not intended for use in diagnostic procedures.

*cFluor™ R668 is equivalent to CF^®647 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.