The eBioRDR5 monoclonal antibody binds to human CD127, a type I glycoprotein with a molecular weight of 75-80 kD, also known as Interleukin-7 Receptor-alpha. CD127 complexes with CD132 (common gamma chain), to form the multi-functional IL-7 receptor (IL-7R). CD127 is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways, including the Jak/STAT pathway. IL-7 is indispensable for the development of lymphocytes and for the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is known to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells. CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs).

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: eBioRDR5 

Specificity: CD127 

Reactivity: Human, non-human primate, Rhesus monkey, mouse

Format: cFluor^® R659

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Sudo T, et al. 1993. Proc Natl Acad Sci USA. 90:9125-9129.
2. He YW and Malek TR. 1998. Crit Rev Immunol. 18:503-524.
3. Huster KM, et al. 2004. Proc Natl Acad Sci USA. 101:5610-5615.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403-2408.
5. Morrissey PJ, et al. 1989. J Exp Med. 169:707-716.
6. Liu W, et al. 2006. J Exp Med. 203:1701-1711.

For research use only. Not intended for use in diagnostic procedures.