cFluor® B520 Anti-Human CD4 (SK3)
The SK3 monoclonal antibody binds to human CD4, a 59-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD4 molecule is expressed predominantly on thymocytes and a subpopulation of mature T-helper lymphocytes. It is also present on monocytes at low levels(1) (3). CD4 plays a role in cell-cell interaction by acting as a co-receptor for MHC class II in antigen recognition. CD4 also has been shown to bind glycoprotein 120 on external envelope of HIV(2). The antibody was conjugated to a fluorophore and purified by affinity chromatography.
Format: cFluor® B520
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
- Evans RL, et al. 1981. Immunol. 78:544
- Arno A et al. 1999. J. Infect. Dis. 180:56
- Muench M, et al. 1997. Blood 89:1364
These products are for research use only. Not intended for use in diagnostic procedures.