The SK3 monoclonal antibody binds to human CD4, a 59-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. The CD4 molecule is expressed predominantly on thymocytes and a subpopulation of mature T-helper lymphocytes. It is also present on monocytes at low levels. CD4 plays a role in cell-cell interaction by acting as a co-receptor for MHC class II in antigen recognition. CD4 also has been shown to bind glycoprotein 120 on external envelope of HIV2. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  SK3

Reactivity:          Human

Format:               cFluor® BYG781¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).

Data Sheets:       IFU SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Evans RL, et al. 1981. Immunol. 78:544
2. Arno A et al. 1999. J. Infect. Dis. 180:56
3. Muench M, et al. 1997. Blood 89:1364

This product is intended for in vitro diagnostic use to identify human cells expressing CD4 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.

¹cFluor® BYG781 is a tandem dye made with R-PE. Caution: Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.