The WM15 monoclonal antibody binds to CD13, a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase is expressed as a homodimer on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and on a subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 acts as the cellular receptor for Coronavirus. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone: WM15

Reactivity: Human, Baboon, Chimpanzee, Cotton-topped Tamarin

Format: cFluor^® BYG750¹

Isotype: Mouse IgG1, κ

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze.

Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets: TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Saiki I, et al. 1993. Int J Cancer. 54:137.
3. Rosenzwajg M, et al. 2000. Blood 95:453.
4. Kawase M, et al. 2008. J Virol. 83:712.

¹cFluor^® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

For Research Use Only. Not intended for use in diagnostic procedures.