The UP-H2 monoclonal antibody binds to human CD300e, also known as IREM-2 (immune receptor expressed by myeloid cells 2), CMRF35-A5, LMIR6, or CD300Le. CD300e is a 34 kD type I single transmembrane glycoprotein and is a member of the CD300 family and of the Ig-superfamily. CD300e is expressed on monocyte and myeloid dendritic cells, but not on granulocytes and lymphoid dendritic cells. CD300e is an activating receptor through interaction with DAP12.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: UP-H2

Specificity: CD300e 

Reactivity: Human

Format: cFluor^® BYG750¹

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Aguilar H, et al. 2004. J. Immunol. 173:6703

For research use only. Not intended for use in diagnostic procedures.

¹cFluor^® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.