The HEK/1/85a monoclonal antibody binds to human CD195 is a 45 kD G protein-coupled transmembrane chemokine receptor, also known as CCR5. CD195 binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation, and has been shown to act as a co-receptor for R5 HIV-1 cell entry. Modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:          HEK/1/85a

Reactivity:     Human

Format:          cFluor^® BYG750¹

Isotype:            Rat IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Samson M, et al. 1996. Biochemistry 35:3362-3367.
2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161-17166.
3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147-152.
4. Deng H, et al. 1996. Nature 381:661-666.
5. Mañes S, et al. 2003. J. Exp. Med. 198:1381-1389.
6. Vaday GG, et al. 2006. Prostate 66:124-134.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

¹cFluor^® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.