cFluor® BYG750 Anti-Human CD19 (HIB19)
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Description
The HIB19 monoclonal antibody binds to human CD19, a 95-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. CD19 forms a signal transduction complex with the complement receptor 2 (CD21), a tetraspanin membrane protein, TAPA-1 (CD81), and Lue 13 to function as a dominant signaling component on the surface of B cells1,2. It is expressed in all phases of B cell development, maturation and differentiation except at the terminal stage of differentiation, lost in plasma cells3. It is also present on follicular dendritic cells and absent on T cells. The antibody was conjugated to a fluorophore and purified by chromatography.
PRODUCT DETAILS
Clone: HIB19
Reactivity: Human
Format: cFluor® BYG7501
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Wang K, et al. 2012. Exp Hematol Oncol. 10.1186/2162-3619-1-36
- Bradbury LE, et al. 1993. J Immunol. 151(6):2915-2927
- Nadler LM, et al. 1983. J Immunol. 131(1):244-250
These products are for research use only. Not intended for use in diagnostic procedures.
1cFluor® BYG750 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.