cFluor® BYG710 Anti-Human IgD (IgD26)
View Cart or Continue shopping.
Description
The 4A11 monoclonal antibody binds to the Fab fragment of human IgG. IgG is a membrane BCR that is produced when activated naïve B cells undergo class switch recombination of the Ig heavy chain constant region. This class-switching results in IgM and IgD being replaced by IgG as the activated B cell develops into memory and antibody secreting cells. On memory B cells, when IgG binds to its target antigen, it initiates signaling and activation that rapidly leads to proliferation and differentiation into antibody secreting cells. The antibody was conjugated to a fluorophore and purified by chromatography.
PRODUCT DETAILS
Clone: IgD26
Reactivity: Human
Format: cFluor® BYG710
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
- Chen K, et al. 2009. Nat. Immunol. 10:889.
- Lee CH, et al. 2005. J. Exp. Med. 203:63.
- Sutter JA, et al. 2008. Clin. Immunol. 126:282.
These products are for research use only. Not intended for use in diagnostic procedures.