The 29E.2A3 monoclonal antibody binds to human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

PRODUCT DETAILS

Clone:       29E.2A3

Reactivity:   Human

Format:          cFluor^® BYG710¹ 

Isotype:       Mouse IgG2b, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:      TDS SDS

RECOMMENDED USAGE 

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Freeman GJ, Long AJ, Iwai Y, et al. 2000. J Exp Med. 192:1027-1034.
2. Latchman Y, Wood CR, Chernova T, et al. 2001. Nat Immunol. 2(3):261-268.
3. Bennett F, Luxenberg D, Ling V, et al. 2003. J Immunol. 170:711-718.
4. Brown JA, Dorfman DM, Ma FR, et al. 2003. J Immunol. 170:1257-1266.
5. Carter L, Fouser LA, Jussif J, et al. 2002. Eur J Immunol. 32:634-643.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

¹cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.