The 104D2 monoclonal antibody binds to human CD117, a 145-kDa protein tyrosine kinase. CD117, also known as c-kit, is the receptor for the hematopoietic cytokine stem cell factor (SCF) expressed on hematopoietic progenitor cell subsets, thymocytes, mast cells hepatocytes and histiocytes. It is also commonly expressed on acute myeloid leukemias (AML). When bound to stem cell factor (c-kit ligand), CD117 activates tyrosine kinase and is involved in cell survival, proliferation and differentiation. The antibody is conjugated to a fluorophore and purified by chromatography

PRODUCT DETAILS

Clone:                  104D2

Reactivity:         Human, Cynomolgus, Bovine

Format:               cFluor^® BYG710*

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Simmons PJ, et al. 1994. Exp Hematol. 22(2):157-165.
2. Escribano L, et al. 1998. Leuk Lymphoma. 30(5-1): 459–466.
3. Blume-Jensen P, et al. 1991. EMBO J. 10(13): 4121–4128.

These products are for research use only. Not intended for use in diagnostic procedures.

 *cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.