The 927 monoclonal antibody binds to mouse CD317, also known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1. CD317 is a type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I interferon-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I interferons and interferon-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and on some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:       927

Reactivity:    Mouse

Format:          cFluor^® BYG667¹

Isotype:             Rat IgG2b, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:      TDS SDS

RECOMMENDED USAGE 

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Douglas JL. et al. 2009. J Virol. 83(16):7931-7947.
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603-1614.
3. Neil SJ. et al. 2008. Nature 451:425-430.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

¹cFluor^® BYG667 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.