The OKT9 monoclonal antibody binds to human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. The transferrin receptor is also present on early erythroid cells, but is lost as reticulocytes differentiate into mature erythrocytes. The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:            OKT9

Reactivity:       Human

Format:         cFluor^® BYG667¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Hentze MW, et al. 1996. Proc Natl Acad Sci U S A. 93:8175-82.
2. Trowbridge IS, et al. 1993. Annu Rev Cell Biol. 9:129-61.

These products are for research use only. Not intended for use in diagnostic procedures.

¹ cFluor® BYG667 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.