The 9E2 monoclonal antibody binds to human CD335, also known as NKp46. CD335 is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is directly involved in target cell recognition and lysis, and is exclusively expressed on CD3- CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytotoxicity receptor (NCR) and plays an important role in killing virus-infected tumor cells and cells that are not protected by expression of MHC-class I.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: 9E2 

Specificity: CD335-NKp46 

Reactivity: Human

Format: cFluor^® BYG667¹

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147-1150.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309-331.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656-1666.

For research use only. Not intended for use in diagnostic procedures.

¹cFluor^® BYG667 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.