The CD25-4E3 monoclonal antibody binds human CD25, a 55 kDa type I transmembrane glycoprotein. CD25 is known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on progenitor lymphocytes, activated T & B cells, and monocytes, as well as on regulatory T cells^(1-4). When CD25 is in association with CD122, IL-2 receptor beta chain and with CD132, the common gamma chain, the resulting complex is the high-affinity IL-2 receptor. IL-2 receptor signaling is key in lymphocyte proliferation and survival⁽⁵⁾.

The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone: CD25-4E3

Specificity: CD25

Reactivity: Human, Non-human Primate

Format: cFluor^® BYG645^{1, 2}

Isotype: Mouse IgG2b, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. 

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Liao W, et al. Curr Op Immunol. 23, 598 (2011)
2. Sakaguchi S, et al. J Immunol. 155, 1151 (1995)
3. Brisslert M, et al. Immunology. 117, 548 (2006)
4. Scheibenbogen C, et al. Research in Immunology. 143, 33 (1992)
5. Ross SH & Cantrell DA. Annu Rev Immunol. 36, 411 (2018)
6. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

For research use only. Not intended for use in diagnostic procedures.

¹cFluor BYG645^® is equivalent to RPE-CF^®620R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

²cFluor BYG645^® is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.