The SK1 monoclonal antibody binds to human CD8a, a 32-34-kDa type I glycoprotein that forms either as an α/α homodimer α/β heterodimer with its isoform CD8b. CD8 is found on thymocytes, a subset of peripheral blood T cells, majority expressing α/β heterodimer, and NK cells, majority expressing α/α homodimer¹. CD8 plays a role in antigen recognition and class I MHC-restricted T cell development. CD8 functions are associated with two domains of CD8a. CD8a ectodomain binding to the α3 domain of MHC class I increases the adhesion between CD8⁺ T lymphocytes and target cells. The cytoplasmic tail binding to the tyrosine kinase p56^lck contributes to signal transduction and T cell activation^2,3. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  SK1

Reactivity:       Human, African Green, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey

Format:              cFluor® BYG610¹²

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).

Data Sheets:       IFU SDS

REFERENCES

1. Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8
2. Kern Petra S. et al. 1998. Immunity. 519-530
3. Veillette A. et al. 1988. Cell. 55:301-308

This product is intended for in vitro diagnostic use to identify human cells expressing CD8 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.

¹cFluor® BYG610 Anti-Human CD8 (SK1) is manufactured and supplied by BioLegend, Inc. and is commercialized under the trademark PE-Dazzle 594™.

²cFluor® BYG610 is a tandem dye made with R-PE. Caution: Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.