The mouse monoclonal antibody clone M5E2 binds to human CD14, a 53-55-kDa glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that works as a receptor on myeloid cells for ligands such as lipopolysaccharide (LPS). CD14 is a receptor that binds to complexes of LPS and LBP with high affinity. It is expressed on monocytes and macrophages at high levels. It is also present in some interfollicular dendritic cells, macrophages, reticular dendritic cells, and Langerhans cells.

PRODUCT DETAILS

Clone: M5E2

Specificity: CD14 

Reactivity: Human, Capuchin monkey, Chimpanzee, Common Marmoset, Cotton-Topped Tamarin, Cow, Cynomolgus macaque, Dog, Pigtailed Macaque, Rhesus, Squirrel monkey

Format: cFluor^® BYG610^{1, 2}

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Pugin J, et al. 1998. Infect Immun. 66:1174
2. Wright SD, et al. 1990. Science. 249:1431

For research use only. Not intended for use in diagnostic procedures.

¹ cFluor^® BYG610 is equivalent to RPE-CF^®596R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

² cFluor^® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.