The mouse monoclonal antibody clone BC96 binds to human CD25 (IL-2 receptor alpha, IL-2Rα) is a 55 kDa surface protein that serves as the low-affinity receptor for interleukin-2 (IL-2). While CD25 can bind IL-2 alone without inducing signaling, it is primarily found as part of the high-affinity IL-2 receptor complex, along with CD122 (IL-2 receptor beta) and CD132 (common gamma chain). CD25 is expressed during T and B cell development, on activated T and B cells, macrophages, and at high levels on natural regulatory T cells (Tregs) in the thymus and periphery, as well as on induced peripheral Tregs. IL-2 receptor signaling is involved in lymphocyte proliferation, survival, and differentiation, influencing Th17 and Treg cell function. CD25 also plays a role in dendritic cell activity and is a marker for Hodgkin’s disease and autoimmune conditions such as Type I diabetes.

PRODUCT DETAILS

Clone: BC96

Specificity: CD25

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Macaque

Format: cFluor^® BYG575

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Knapp W., et al., ed. Leucocyte typing IV. 1989.Oxford University Press
2. Schlossman SF. Stuart F. Schlossman, et al, 1995. ed. Leucocyte typing V : white cell differentiation antigens Oxford: Oxford University Press.
3. Chapel A, Bensussan A, et al, 1992. J Virol:66:3966-70.

For research use only. Not intended for use in diagnostic procedures.