The NK-1 monoclonal antibody binds to human CD57, a 100-115 kD oligosaccharide antigenic determinant also known as HNK-1, NK-1, and Leu-7. CD57 occurs on a variety of proteins, lipids, and chondroitin sulfate proteoglycans, and is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8 T cells. CD57 is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is not known, binding of the marker to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances, including AIDS, autoimmune disease, viral infections, and allograft transplants.

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: NK-1 

Specificity: CD57 

Reactivity: Human

Format: cFluor^® B548^*

Isotype: Mouse IgM, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. PBMCs may require a lower volume for optimal staining when used in combination with other reagents. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed). Oxford University Press, Oxford pp 711- 714.
2. Schachner M, et al. 1995. Trends Neurosci. 18:183-191.
3. Miura R, et al. 2001. J Neurochem. 76:413-424.
4. Palmer BE, et al. 2005. J. Immunol. 175:8415-8423.
5. Wood KL, et al. 2005. Clin. Immunol. 117:294-301.
6. Bentebibel S, et al. 2011. Proc Natl Acad Sci USA 108:E488-E497

For research use only. Not intended for use in diagnostic procedures.

^*cFluor^® B548 is equivalent to CF^®514 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.