The HI98 monoclonal antibody binds to human CD15, a 220-kDa carbohydrate structure. CD15 is also known as 3-fucosyl-N-acetyllactosamine (3-FAL), Lewis X, 3-FAL, X-hapten, and SSEA-1¹. CD15 is highly expressed on granulocytes, including neutrophils and eosinophils, and some on monocytes. It is absent from lymphocytes or basophils. CD15, a marker for human myeloid cell, is involved in various cell functions including phagocytosis, bacterial activity, neutrophil adhesion to dendritic cells and chemotaxis². The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  HI98

Reactivity:          Human, Chimpanzee

Format:          cFluor® B548¹

Isotype:               Mouse IgM, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).

Data Sheets:       IFU SDS

REFERENCES

1. Gadhoum SZ, et al. 2008. Nat Chem Biol. 4(12):751-757.
2. Lund-Johansen F, et al. 1992. J Immunol. 148(10):3221-3229.

This product is intended for in vitro diagnostic use to identify human cells expressing CD15 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.

 ¹cFluor® B548 is equivalent to CF®514 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel