The HI98 monoclonal antibody binds to human CD15, a 220-kDa carbohydrate structure. CD15 is also known as 3-fucosyl-N-acetyllactosamine (3-FAL), Lewis X, 3-FAL, X-hapten, and SSEA-1¹. CD15 is highly expressed on granulocytes, including neutrophils and eosinophils, and some on monocytes. It is absent from lymphocytes or basophils. CD15, a marker for human myeloid cell, is involved in various cell functions including phagocytosis, bacterial activity, neutrophil adhesion to dendritic cells and chemotaxis². The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  HI98

Reactivity:          Human, Chimpanzee

Format:                cFluor® B548*

Isotype:               Mouse IgM, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Gadhoum SZ, et al. 2008. Nat Chem Biol. 4(12):751-757.
2. Lund-Johansen F, et al. 1992. J Immunol. 148(10):3221-3229.

These products are for research use only. Not intended for use in diagnostic procedures.

*cFluor™ B548 is equivalent to CF®514 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.