cFluor® B515 Anti-Human CD5 (L17F12)
The L17F12 monoclonal antibody binds to human CD5, a 67-kDa single chain type I glycoprotein in the scavenger receptor family (1)(2). CD5, also known as Leu-1, Ly-1 or T1, is expressed on thymocytes, T cells and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction (3). The antibody was conjugated to a fluorophore and puriﬁed by afﬁnity chromatography.
Format: cFluor® B515*
Isotype: Mouse IgG2a, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
- Engleman EG, et al. 1981. Proc Natl Acad Sci USA. 78(3):1791-5
- Velasco-de Andrés, María et al. 2020. Cells vol. 9,12 2589
- Tabbekh, Mouna et al. 2013. Oncoimmunology 2,1
These products are for research use only. Not intended for use in diagnostic procedures.
*cFluor® B515 is equivalent to CF®488A manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.