The ICRF44 monoclonal antibody reacts with human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b associated with integrin β2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

PRODUCT DETAILS

Clone: ICRF44

Specificity: CD11b

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Monkey

Format: cFluor^® B515^*

Isotype: Mouse IgG1, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
3. Marsik C, et al. 2003. Shock 20:493.
4. Charles N, et al. 2010. Nat. Med. 16:701.
5. Thurlow LR, et al. 2010. Infect. Immun. 128:1128.
6. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
7. Wen T, et al. 2014. J Immunol. 192:5481.

For research use only. Not intended for use in diagnostic procedures.

^*cFluor^® B515 is equivalent to CF^®488A manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.