Description: The A2MR-a2 monoclonal antibody reacts with human CD91, also known as LRP-1 and alpha-2 macroglobulin receptor. CD91 is a 600 kDa, type 1 transmembrane protein consisting of an alpha and beta chain. The alpha chain interacts with multiple structurally and functionally diverse ligands and the beta chain contains 2 tyrosine phosphorylation motifs capable of interacting with adaptor and signaling proteins. Binding of ligands mediates ligand endocytosis and trafficking to lysosomes. This receptor is expressed by cells in the monocytic lineage, dendritic cells, hepatocytes, firbroblasts, adipocytes, neurons, and syncytiotrophoblast. CD91 is involved in cross-presenting antigens via MHC class I molecules on antigen presenting cells after endocytosis of heat shock protein-peptide complexes. It also plays a role in cell migration and is involved in activating signaling cascades.

Applications Reported: This A2MR-a2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A2MR-a2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells. Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow) Laser