CD74 Monoclonal Antibody (5-329), Super Bright™ 436, eBioscience™
Isotype: IgG1, kappa
Format: Super Bright™ 436
Application: Flow Cytometry
Tested Dilution: 5 µL (0.5 µg)/test
Concentration: 5 µL/Test
Storage: 4° C, store in dark, DO NOT FREEZE!
Formulation: PBS, pH 7.2, containing 0.09% sodium azide
Purification: Affinity chromatography
Data Sheet: TDS
Description: This 5-329 monoclonal antibody recognizes human CD74, also known as the MHC Class II-associated invariant chain. A 33-43 kDa nonpolymorphic type II membrane protein, CD74 is highly expressed on B cells and subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells. Monocytes and macrophages also express CD74, but at lower levels. CD74 acts as a chaperone of MHC Class II (HLA-DR) proteins, promoting their translocation from the ER to endocytic compartments during antigen presentation. Activation of NFkappaB and ERK has been shown following CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells. CD74 has also been demonstrated to interact with CXCR2 and CXCR4.
Applications Reported: This 5-329 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5-329 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.