PRODUCT DETAILS

Host: Mouse

Isotype: IgG2a

Clonality: Monoclonal

Clone: CL2

Format: FITC

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 10 μL/Test

Storage: 4°C, store in dark

Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: E-Selectin (CL-2) is a mouse monoclonal antibody raised against E-Selectinof human origin.

CL2, but not CL37, blocks binding of leukocytes to E-selectin.

. Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules. Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells.

Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail.

Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation. Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation.

Applications Tested: Flow Cytometry.

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.