PRODUCT DETAILS

Host: Armenian Hamster

Isotype: IgG

Clonality: Monoclonal

Clone: 1C2

Format: PE

Reactivity: Ms, Rt

Application: Flow Cytometry

Tested Dilution: 1 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The monoclonal antibody 1C2 recognizes mouse and rat CD42d, a single transmembrane protein also known as platelet glycoprotein V (GPV). CD42d interacts with CD42a, CD42b and CD42c to form the receptor for von willebrand factor and thrombin. This functional complex plays a role in platelet adhesion. Expression of CD42d (unlike other markers such as CD41) is restricted to resting platelets and megakaryocytes and therefore is a good marker for development of megakaryocytes.

Applications Reported: This 1C2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1C2 antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.