PRODUCT DETAILS

Host: Rat

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: GK1.5

Format: FITC

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.