PRODUCT DETAILS

Host: Rat

Isotype: IgG2a, kappa

Clonality: Monoclonal

Clone: eBioTR23 (TR23)

Format: PE

Reactivity: Ms

Application: Flow Cytometry

Tested Dilution: 0.5 µg/test

Concentration: 0.2 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The eBioTR23 monoclonal antibody reacts with mouse Toll-like receptor 1 (TLR1, CD281). TLR1 is an approximately 98 kDa protein belonging to the Toll-like receptor family, and is closely-related to mouse TLR6 in both structure and function. Through the formation of a complex with TLR2, TLR1 is able to bind bacterially-derived triacyl lipopeptides, as well as soluble factors released from Neisseria meningitidis. TLR1 is expressed by macrophages and macrophages in TLR1-deficient mice display impaired production of inflammatory cytokines upon stimulation with triacyl lipopeptides and lipoproteins from mycobacteria.

Applications Reported: This eBioTR23 (TR23) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioTR23 (TR23) antibody has been tested by flow cytometric analysis of A20 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.