This L161 monoclonal antibody detects CD1c (also known as BDCA-1), a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms).

Applications Reported: This L161 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This L161 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Our internal testing shows that NovaFluor Yellow 730 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells. Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 552 nm; Emission: 730 nm; Laser: 561 nm (Yellow) Laser