![C57Bl/6 splenocytes were stimulated with Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) (TNB-4970) supplemented with Brefeldin A Solution (TNB-4506) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with PE Anti-Mouse IL-2 (50-7021) (right panel) or PE Rat IgG2b (left panel).](http://cytekbio.com/cdn/shop/products/TNB-4970_large.png?v=1675104757)
Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X)
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Description
Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate) and a calcium ionophore (Ionomycin). This Cell Stimulation Cocktail (w/o protein transport inhibitors) (500X) can be used to elicit cytokine production in a variety of cell types allowing for detection in immunoassays.