Brefeldin A Solution (1000X)
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Brefeldin A (BFA) is a protein transport inhibitor that can be added to cell culture medium during in vitro activation of cells to inhibit the secretion of cytokines elicited by stimulation. Addition of BFA leads to leads to an accumulation of newly synthesized protein in the Golgi complex. The use of Brefeldin A effectively enhances the detection of most mouse and human intracellular cytokines however, other protein transport inhibitors such as Monensin can also be used. It is recommended that investigators evaluate the optimal reagent to use for their specific assay system.
Teghanemt A, Pulipati P, Misel-Wuchter K, Day K, Yorek MS, Yi R, Keen HL, Au C, Maretzky T, Gurung P, Littman DR, Issuree PD. CD4 expression in effector T cells depends on DNA demethylation over a developmentally established stimulus-responsive element. Nat Commun. 2022 Mar 18;13(1):1477. doi: 10.1038/s41467-022-28914-4. PMID: 35304452.
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Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.
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