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C57Bl/6 splenocytes were stimulated with PMA and Ionomycin and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with 0.25 ug Biotin Anti-Mouse IFN gamma (30-7311) (right panel) or 0.25 ug Biotin Rat IgG1 isotype control

Biotin Anti-Mouse IFN gamma (XMG1.2)

SKU 30-7311-U025
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The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).

The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. This format can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of ≥≥IFN-g in a variety of assays (use format suitable for functional assays).

Name Biotin Anti-Mouse IFN gamma (XMG1.2)
Cat. No. 30-7311
Alternative Names IFN-g, IFNg, Interferon-g, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Gene ID 15978
Clone XMG1.2
Isotype Rat IgG1, kappa
Reactivity Mouse
Format Biotin
Application Flow Cytometry
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