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C57Bl/6 splenocytes were stained with APC Anti-Mouse CD4 (20-0041) and 0.125 ug Biotin Anti-Mouse CD25 (30-0251) (right panel) or 0.125 ug Biotin Rat IgG1 isotype control (left panel) followed by Streptavidin PE.

Biotin Anti-Mouse CD25 (PC61.5)

SKU 30-0251-U025
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The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.

The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25-. This clone has also been reported for depletion of Treg cells in vivo (use format suitable for functional assays).


Recent Publications:

Castillo-González R, Cibrian D, Fernández-Gallego N, Ramírez-Huesca M, Saiz ML, Navarro MN, Fresno M, Fuente H, Sánchez-Madrid F. Galectin-1 expression in CD8+ T lymphocytes controls inflammation in contact hypersensitivity. J Invest Dermatol. 2020 Nov 9:S0022-202X(20)32281-8. doi: 10.1016/j.jid.2020.10.020. PMID: 33181141.

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Name Biotin Anti-Mouse CD25 (PC61.5)
Cat. No. 30-0251
Alternative Names Interleukin-2 Receptor alpha, IL-2R alpha, Ly-43, p55, Tac
Gene ID 16184
Clone PC61.5
Isotype Rat IgG1, lambda
Reactivity Mouse
Format Biotin
Application Flow Cytometry
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