The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

PRODUCT DETAILS

Clone: AFP-01

Specificity: Alpha-Fetoprotein

Reactivity: Human

Format: Biotin

Isotype: Mouse IgG1

Application: ICC, ELISA, IP

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.

REFERENCES

1. Stefanová I, et al. 1988. J Immunol Methods 111:67-73.
2. Cavin LG, et al. 2004. Cancer Res 64:7030-8.
3. Green CJ, et al. 2017. Cell Tissue Bank 18:597-604.
4. Colquhoun DR, et al. 2009. Environ Health Perspect 117:832-8.
5. Wang Z, et al. 2019. Mol Med Rep 19:4623-4630.

For research use only. Not intended for use in diagnostic procedures.