PRODUCT DETAILS

Host: Mouse

Isotype: IgG1, kappa

Clonality: Monoclonal

Clone: H74

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: 0.25 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x. Cross reactivity of this antibody to other proteins has not been determined. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand. H74 stains human B7-H4 transfected cells and not peripheral blood cells. Exact expression pattern of B7-H4 has not been fully characterized.

Applications Reported: This H74 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.