The mouse monoclonal antibody clone TB28.2 binds to human immunoglobulin light chain, kappa (κ), a 22.5 kDa polypeptide subunit of an antibody. There are two types of immunoglobulin light chains in human: kappa (κ) and lambda (λ). Under normal conditions, antibodies produced by B cells only express one type of light chain and ratio of kappa to lambda is approximately 2:1.

PRODUCT DETAILS

Clone: TB28.2

Specificity: Ig Kappa

Reactivity: Human

Format: APC-Cyanine7

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. P Gasser, et al. 2020. Nature Comm, vol. 11(1):165.
2. Vaunchy Gamonet, et al. 2015. Intl J Cancer, vol. 137(1):116-26.
3. Pierguido et al. 2016. European journal of medical genetics, vol. 59(4): 219-22.
4. Jerry A Katzmann, et al. 2002. Clinical Chemistry, vol. 48(9): 1437–1444.

For research use only. Not intended for use in diagnostic procedures.

APC-Cyanine7 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.