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Human peripheral blood lymphocytes were stained with 5 uL (0.125 ug) APC-Cy7 Anti-Human CD8a (25-0088) (solid line) or 0.125 ug APC-Cy7 Mouse IgG1 isotype control (dashed line).

APC-Cyanine7 Anti-Human CD8a (RPA-T8)

SKU 25-0088-T025
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The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.

Recent Publications:

Lee J, Sheen JH, Lim O, Lee Y, et al.  Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy. Sci Rep. 2020 Oct 20;10(1):17753. doi: 10.1038/s41598-020-74772-9. PMID: 33082438; PMCID: PMC7576162.   

» View on Scientific Reports

Name APC-Cyanine7 Anti-Human CD8a (RPA-T8)
Cat. No. 25-0088
Alternative Names CD8 alpha, leu-2a
Gene ID 925
Clone RPA-T8
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Format APC-Cyanine7
Application Flow Cytometry
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