The mouse monoclonal antibody clone 9F10 binds to human CD49d, a 150 kDa integral membrane protein known as integrin alpha4 (ITGA4). Together with integrin beta1 (CD29), CD49d forms the VLA-4 heterodimer that serves as a receptor for fibronectin and VCAM-1. VLA-4 is widely expressed on lymphocytes, monocytes, thymocytes, and NK cells. CD49d can also dimerize with integrin beta7 to form a receptor that binds the mucosal vascular addressin molecule, MAdCAM-1. CD49d is involved in cellular adhesion to other cells and the extracellular matrix. CD49d was also studied as a prognostic marker for disease progression and treatment options for chronic lymphocytic leukemia (CLL) and Duchenne muscular dystrophy (DMD).

PRODUCT DETAILS

Clone: 9F10

Specificity: CD49d

Reactivity: Human

Format: APC-Cyanine7

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.

For research use only. Not intended for use in diagnostic procedures.

APC-Cyanine7 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.