The antibody MEM28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

PRODUCT DETAILS

Clone: MEM28

Specificity: CD45

Reactivity: Human

Format: APC-Cyanine7

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Li FJ, et al. 2005. Blood 105:3295-302.
2. Cosenza-Nashat MA, et al. 2006. Brain Pathol 16:256-65.
3. Dawes R, et al. 2006. J Immunol 176:3417-25.
4. Bijian K, et al. 2007. Mol Immunol 44:3682-90.
5. Desharnais P, et al. 2008. Apoptosis 13:197-212.
6. Horejsí V, et al. 1988. Folia Biol (Praha) 34:23-34.
7. Ilangumaran S, et al. 1998. Blood 91:3901-8.
8. Drbal K, et al. 2007. Int Immunol 19:675-84.
9. Majer F, et al. 2012. Gene 498:183-95.

For research use only. Not intended for use in diagnostic procedures.

APC-Cyanine7 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.