The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.

PRODUCT DETAILS

Clone: G28-8

Specificity: CD180

Reactivity: Human

Format: APC-Cyanine7

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Kikuchi Y, et al. 2001. Ann Rheum Dis 60:1137-40.
2. Divanovic S, et al. 2007. J Leukoc Biol 82:265-71.
3. Yazawa N, et al. 2003. Blood 102:1374-80.
4. Divanovic S, et al. 2005. Nat Immunol 6:571-8.
5. Valentine MA, et al. 1988. J Immunol 140:4071-8.
6. Clark EA, et al. 1989. J Immunol 143:3873-80.

For research use only. Not intended for use in diagnostic procedures.

APC-Cyanine7 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.