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C57Bl/6 splenocytes were stained with FITC Anti-Mouse CD4 (35-0041), followed by intracellular staining with 0.125 ug APC Anti-Mouse Foxp3 (20-5773) (right panel) or 0.125 ug APC Mouse IgG1 isotype control (left panel).

APC Anti-Mouse Foxp3 (3G3)

SKU 20-5773-U025
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The 3G3 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted, and upon TCR activation is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce expression of Foxp3 in naïve T cells, driving their development into Foxp3+ Tregs, which are called “induced” or “adaptive” Tregs. These cells are phenotypically similar to so-called “natural” Tregs (CD4+ CD25high Foxp3+) which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity.

It is important to review the literature in choosing an antibody for the Foxp3 antigen in flow cytometry, as the potential for high background or non-specific staining may be observed. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.


Recent Citations:

Núñez-Ruiz A, Sánchez-Brena F, López-Pacheco C, Acevedo-Domínguez NA, Soldevila G. Obesity modulates the immune macroenvironment associated with breast cancer development. PLoS One. 2022 Apr 26;17(4):e0266827. doi: 10.1371/journal.pone.0266827. PMID: 35472214; PMCID: PMC9041840.

» View on PubMed

Name APC Anti-Mouse Foxp3 (3G3)
Cat. No. 20-5773
Alternative Names Forkhead Box P3, Scurfin
Gene ID 50943/20371
Clone 3G3
Isotype Mouse IgG1, kappa
Reactivity Mouse
Cross Reactivity Rhesus macaque
Format APC
Application Flow Cytometry
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