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C57Bl/6 bone marrow cells were stained with FITC Anti-Mouse CD11b (35-0112) and 0.5 ug APC Anti-Mouse F4/80 Antigen (20-4801) (right panel) or 0.5 ug APC Rat IgG2b isotype control (left panel).

APC Anti-Mouse F4/80 Antigen (BM8.1)

SKU 20-4801-U025
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The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Recent Publications:

Taniguchi H, Caeser R, Chavan SS, Zhan YA, Chow A, Manoj P, Uddin F, Kitai H, Qu R, Hayatt O, Shah NS, Quintanal Villalonga Á, Allaj V, Nguyen EM, Chan J, Michel AO, Mukae H, de Stanchina E, Rudin CM, Sen T. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022 May 17;39(7):110814. doi: 10.1016/j.celrep.2022.110814. PMID: 35584676.

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Tan X, Banerjee P, Shi L, Xiao GY, Rodriguez BL, Grzeskowiak CL, Liu X, Yu J, Gibbons DL, Russell WK, Creighton CJ, Kurie JM. p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Sci Adv. 2021 Jun 18;7(25):eabf4885. doi: 10.1126/sciadv.abf4885. PMID: 34144984.

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Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020).

Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396.

Wu J and Waxman DJ. 2015. OncoImmunol. DOI:10.1080/2162402X.2015.1005521. (Flow Cytometry)

Name APC Anti-Mouse F4/80 Antigen (BM8.1)
Cat. No. 20-4801
Alternative Names EMR1, Ly71
Gene ID 13733
Clone BM8.1
Isotype Rat IgG2b, κ
Reactivity Mouse
Format APC
Application Flow Cytometry
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