APC Anti-Mouse CD25 (PC61.5)
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The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.
The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25-. This clone has also been reported for depletion of Treg cells in vivo (use format suitable for functional assays).
Brandi P, Conejero L, Cueto FJ, Martínez-Cano S, Dunphy G, Gómez MJ, Relaño C, Saz-Leal P, Enamorado M, Quintas A, Dopazo A, Amores-Iniesta J, Del Fresno C, Nistal-Villán E, Ardavín C, Nieto A, Casanovas M, Subiza JL, Sancho D. Trained immunity induction by the inactivated mucosal vaccine MV130 protects against experimental viral respiratory infections. Cell Rep. 2022 Jan 4;38(1):110184. doi: 10.1016/j.celrep.2021.110184. PMID: 34986349.
Martinez-Lopez M, Iborra S, Conde-Garrosa R and Sancho D. 2014. Eur J Immunol. doi: 10.1002/eji.201444651. (Flow Cytometry)
|Name||APC Anti-Mouse CD25 (PC61.5)|
|Alternative Names||Interleukin-2 Receptor alpha, IL-2Rα, Ly-43, p55, Tac|
|Isotype||Rat IgG1, lambda|