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C57Bl/6 splenocytes were stained with 0.25 ug APC Anti-Hu/Mo CD45R (B220) (20-0452) (solid line) or 0.25 ug APC Rat IgG2a isotype control (dashed line).

APC Anti-Human/Mouse CD45R (B220) (RA3-6B2)

SKU 20-0452-U025
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$27.00

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Description

TDS | SDS

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Recent Publications:
Qiumei Du, Ashley R. Hoover, Igor Dozmorov, Ondine B. Cleaver,Joshua T. Mendell, Nicolai S.C. van Oers. MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012

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Yang BH, Wang K, Wan S, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27(12):3629-3645.e6. doi:10.1016/j.celrep.2019.05.061.

Shimada K, Porritt RA, Markman JL, et al. T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation. Immunity. 2018;49(5):873-885.e7. doi:10.1016/j.immuni.2018.08.022.

Itoh-Nakadai A, Hikota R, Muto A, Kometani K, Watanabe-Matsui M, Sato Y, Kobayashi M, Makamura A et al. 2014. Nat Immunol. 15(12): 1171-1180. (Flow Cytometry)

Name APC Anti-Human/Mouse CD45R (B220) (RA3-6B2)
Cat. No. 20-0452
Alternative Names Ly-5, Lyt-4, T200
Gene ID 19264
Clone RA3-6B2
Isotype Rat IgG2a, κ
Reactivity Human, Mouse
Cross Reactivity Feline
Format APC
Application Flow Cytometry
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