APC Anti-Human/Mouse CD45R (B220) (RA3-6B2)
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Description
The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.
The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
Recent Publications:
Qiumei Du, Ashley R. Hoover, Igor Dozmorov, Ondine B. Cleaver,Joshua T. Mendell, Nicolai S.C. van Oers. MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012
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Yang BH, Wang K, Wan S, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27(12):3629-3645.e6. doi:10.1016/j.celrep.2019.05.061.
Shimada K, Porritt RA, Markman JL, et al. T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation. Immunity. 2018;49(5):873-885.e7. doi:10.1016/j.immuni.2018.08.022.
Itoh-Nakadai A, Hikota R, Muto A, Kometani K, Watanabe-Matsui M, Sato Y, Kobayashi M, Makamura A et al. 2014. Nat Immunol. 15(12): 1171-1180. (Flow Cytometry)
Name | APC Anti-Human/Mouse CD45R (B220) (RA3-6B2) |
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Cat. No. | 20-0452 |
Alternative Names | Ly-5, Lyt-4, T200 |
Gene ID | 19264 |
Clone | RA3-6B2 |
Isotype | Rat IgG2a, κ |
Reactivity | Human, Mouse |
Cross Reactivity | Feline |
Format | APC |
Application | Flow Cytometry |