The mouse monoclonal antibody clone M38 binds to human CD81, a 26 kDa non-glycosylated member of the tetraspanin superfamily (TM4SF). CD81, also known as TAPA-1 (target of an antiproliferative antibody), is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts, and at low levels on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell surface proteins in multimolecular complexes, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

PRODUCT DETAILS

Clone: M38

Specificity: CD81

Reactivity: Human

Format: APC

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127-149.
2. Wright M, et al. 1994. Immunol. Today 15:588-594.
3. Tubita et al. 2019. Sci. Rep. 1:11117-11128.

For research use only. Not intended for use in diagnostic procedures.